How to perform a puncture test using the Mach-1
Equipment:
- https://www.biomomentum.com/accessories/sample-holder-puncture-fixtures/
- https://www.biomomentum.com/accessories/kit-of-spherical-indenters/
- https://www.biomomentum.com/mechanical-testers/mach-1-v500c/
There is no general standard for the puncture test,
you will need to base yourself off published articles for this.
Here is a good starting point where we performed
the puncture of heart valve leaflets:
https://www.biomomentum.com/case-studies/validation-of-a-conditioning-and-rupture-test-method-for-pulmonary-valve-cusps/
Based on this article: https://www.sciencedirect.com/science/article/abs/pii/S1751616112001221?via%3Dihub
The programming looked something like this:
1. Approach indenter
approximately 2mm above the aperture and execute the following sequence to find
the surface of the sample:
Functions
|
Parameters
|
Zero Load
|
N/A
|
Find Contact
|
Stage Axis: Position (z)
Load Cell Axis: Fz
Direction: Positive
Stage Velocity, mm/s: 0.100
Contact Criteria, gf: 10.00
Stage Limit, mm: 10
Stage
Repositioning: None
|
Zero Position
|
N/A
|
2. Execute the following
sequence to perform the pre-conditioning cycles:
Functions
|
Parameters
|
Find Contact
|
Stage Axis: Position (z)
Load Cell Axis: Fz
Direction: Positive
Stage Velocity, mm/s: 0.417 (25 mm/min)
Contact Criteria, gf: 500
Stage Limit, mm: 10
Stage Repositioning: None
Save data as “___.txt”
|
Move Absolute
|
Amplitude, mm: 0.000
Velocity, mm/s: 0.417
Save data as “___.txt”
|
Repetitions
|
3
|
3. Wait 5 minutes for the tissue
to return to its normal state (ensuring the tissue stays hydrated over this
time).
4. Execute this final sequence
to perforate the sample:
Functions
|
Parameters
|
Zero Load
|
N/A
|
Find Contact
|
Stage Axis: Position (z)
Load Cell Axis: Fz
Direction: Positive
Stage Velocity, mm/s: 0.100
Contact Criteria, gf: 10.00
Stage Limit, mm: 10
Stage Repositioning: None
|
Move Relative
|
Amplitude, mm: 8.000
Velocity, mm/s: 0.417
Save data as “___.txt”
|
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