How to perform a puncture test using the Mach-1

How to perform a puncture test using the Mach-1

Equipment: 

  1. https://www.biomomentum.com/accessories/sample-holder-puncture-fixtures/
  2. https://www.biomomentum.com/accessories/kit-of-spherical-indenters/
  3. https://www.biomomentum.com/mechanical-testers/mach-1-v500c/
There is no general standard for the puncture test, you will need to base yourself off published articles for this.


Here is a good starting point where we performed the puncture of heart valve leaflets:
https://www.biomomentum.com/case-studies/validation-of-a-conditioning-and-rupture-test-method-for-pulmonary-valve-cusps/

Based on this article: https://www.sciencedirect.com/science/article/abs/pii/S1751616112001221?via%3Dihub

The programming looked something like this:

1.     Approach indenter approximately 2mm above the aperture and execute the following sequence to find the surface of the sample:

Functions

Parameters

Zero Load

N/A

Find Contact

Stage Axis: Position (z)

Load Cell Axis: Fz

Direction: Positive

Stage Velocity, mm/s: 0.100

Contact Criteria, gf: 10.00

Stage Limit, mm: 10

Stage Repositioning: None

Zero Position

N/A

                        

2.     Execute the following sequence to perform the pre-conditioning cycles:

Functions

Parameters

Find Contact

Stage Axis: Position (z)

Load Cell Axis: Fz

Direction: Positive

Stage Velocity, mm/s: 0.417 (25 mm/min)

Contact Criteria, gf: 500

Stage Limit, mm: 10

Stage Repositioning: None

Save data as “___.txt”

Move Absolute

Amplitude, mm: 0.000

Velocity, mm/s: 0.417

Save data as “___.txt”

Repetitions

3

3.     Wait 5 minutes for the tissue to return to its normal state (ensuring the tissue stays hydrated over this time).

4.     Execute this final sequence to perforate the sample:

Functions

Parameters

Zero Load

N/A

Find Contact

Stage Axis: Position (z)

Load Cell Axis: Fz

Direction: Positive

Stage Velocity, mm/s: 0.100

Contact Criteria, gf: 10.00

Stage Limit, mm: 10

Stage Repositioning: None

Move Relative

Amplitude, mm: 8.000

Velocity, mm/s: 0.417

Save data as “___.txt”


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